› 4fzl

X-ray diffraction
1.46Å resolution

High resolution structure of truncated bacteriocin syringacin M from Pseudomonas syringae pv. tomato DC3000

Released:
DOI: 10.2210/pdb4fzl/pdb
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Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
The crystal structure of the lipid II-degrading bacteriocin syringacin M suggests unexpected evolutionary relationships between colicin M-like bacteriocins.
Grinter R, Roszak AW, Cogdell RJ, Milner JJ, Walker D
J Biol Chem 287 38876-88 (2012)
PMID: 22995910

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-183435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriocin, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 26.73 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88A25 (Residues: 38-276; Coverage: 87%)
Gene name: PSPTO_0572
Sequence domains: Colicin M
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand EDO 13 x EDO
Ligand CL 2 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 55.81Å b: 96.09Å c: 64.27Å
α: 90° β: 114.17° γ: 90°
R-values:
R R work R free
0.154 0.152 0.201
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.
Ghequire et al. (2014)
3 more

4 mentions without citation

The crystal structure of the lipid II-degrading bacteriocin syringacin M suggests unexpected evolutionary relationships between colicin M-like bacteriocins.
Grinter et al. (2012)
3 more

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