› 4g4k

X-ray diffraction
1.52Å resolution

Structure of the Staphylococcus aureus AgrA LytTR Domain

Released:
DOI: 10.2210/pdb4g4k/pdb
PDB entry 4g4k coloured by chain, front view.
PDB entry 4g4k coloured by chain, side view.
PDB entry 4g4k coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Identification of a hydrophobic cleft in the LytTR domain of AgrA as a locus for small molecule interactions that inhibit DNA binding.
Leonard PG, Bezar IF, Sidote DJ, Stock AM
Biochemistry 51 10035-43 (2012)
PMID: 23181972

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Accessory gene regulator protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 12.36 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A0I7 (Residues: 137-238; Coverage: 43%)
Gene names: agr, agrA
Sequence domains: LytTr DNA-binding domain
Structure domains: LytTr DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 41.414Å b: 45.894Å c: 112.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.209
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

8 review citations

Regulation of Virulence in Staphylococcus aureus: Molecular Mechanisms and Remaining Puzzles.
Wang et al. (2016)
7 more

12 mentions without citation

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
11 more