› 4g76

X-ray diffraction
2.38Å resolution

Structure of PaeM, a colicin M-like bacteriocin produced by Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4g76/pdb
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PDB entry 4g76 coloured by chain, front view.
PDB entry 4g76 coloured by chain, side view.
PDB entry 4g76 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Functional and structural characterization of PaeM, a colicin M-like bacteriocin produced by Pseudomonas aeruginosa.
Barreteau H, Tiouajni M, Graille M, Josseaume N, Bouhss A, Patin D, Blanot D, Fourgeaud M, Mainardi JL, Arthur M, van Tilbeurgh H, Mengin-Lecreulx D, Touzé T
J Biol Chem 287 37395-405 (2012)
PMID: 22977250

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid II-degrading bacteriocin Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.45 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1W548 (Residues: 1-289; Coverage: 100%)
Gene names: EXA13, L4V69_26285, paeM
Sequence domains: Colicin M
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 42.812Å b: 72.4Å c: 99.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.17 0.243
Expression system: Escherichia coli

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Citations

2 review citations

Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.
Ghequire et al. (2014)
1 more

3 mentions without citation

The Biology of Colicin M and Its Orthologs.
Chérier et al. (2021)
2 more

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