› 4g98

X-ray diffraction
2.3Å resolution

Rat Heme Oxygenase-1 in complex with Heme and CO at 100K

Released:
DOI: 10.2210/pdb4g98/pdb
PDB entry 4g98 coloured by chain, front view.
PDB entry 4g98 coloured by chain, side view.
PDB entry 4g98 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Discrimination between CO and O(2) in heme oxygenase: comparison of static structures and dynamic conformation changes following CO photolysis.
Sugishima M, Moffat K, Noguchi M
Biochemistry 51 8554-62 (2012)
PMID: 23043644

Function and Biology Details

Reaction catalysed: 
Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme oxygenase 1 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30.61 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06762 (Residues: 1-267; Coverage: 92%)
Gene name: Hmox1
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand CMO 1 x CMO
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 65.974Å b: 65.974Å c: 120.018Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.153 0.218
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent Advances in the Understanding of the Reaction Chemistries of the Heme Catabolizing Enzymes HO and BVR Based on High Resolution Protein Structures.
Sugishima et al. (2020)
5 other articles

2 mentions without citation

Carbon Monoxide and Nitric Oxide as Examples of the Youngest Class of Transmitters.
Nowaczyk et al. (2021)
1 more