› 4gdi

X-ray diffraction
1.95Å resolution

A subtype N10 neuraminidase-like protein of A/little yellow-shouldered bat/Guatemala/164/2009

Released:
DOI: 10.2210/pdb4gdi/pdb
PDB entry 4gdi coloured by chain, front view.
PDB entry 4gdi coloured by chain, side view.
PDB entry 4gdi coloured by chain, top view.
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/164/2009(H17N10))
Primary publication:
Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site.
Zhu X, Yang H, Guo Z, Yu W, Carney PJ, Li Y, Chen LM, Paulson JC, Donis RO, Tong S, Stevens J, Wilson IA
Proc Natl Acad Sci U S A 109 18903-8 (2012)
PMID: 23012478

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (7 distinct):
Neuraminidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 373 amino acids
Theoretical weight: 41.59 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/164/2009(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM85 (Residues: 75-442; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUL, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUL, FUC, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUL, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUL
Carbohydrate polymer
4 bound ligands:
Ligand NAG 13 x NAG
Ligand CA 11 x CA
Ligand GOL 14 x GOL
Ligand NO3 14 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I4
Unit cell:
a: 176.324Å b: 176.324Å c: 193.313Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.223
Expression system: Trichoplusia ni

Quick links

Citations

19 review citations

Interspecies transmission and emergence of novel viruses: lessons from bats and birds.
Chan et al. (2013)
18 more

1 mention without citation

Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site.
Zhu et al. (2012)