› 4gi8

X-ray diffraction
1.95Å resolution

Crystal structure of the MUTB F164L mutant from crystals soaked with the substrate sucrose

Released:
DOI: 10.2210/pdb4gi8/pdb
PDB entry 4gi8 coloured by chain, front view.
PDB entry 4gi8 coloured by chain, side view.
PDB entry 4gi8 coloured by chain, top view.
Source organism: Rhizobium sp. MX-45
Primary publication:
Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity.
Lipski A, Watzlawick H, Ravaud S, Robert X, Rhimi M, Haser R, Mattes R, Aghajari N
Acta Crystallogr D Biol Crystallogr 69 298-307 (2013)
PMID: 23385465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.9 KDa
Source organism: Rhizobium sp. MX-45
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1F3 (Residues: 1-557; Coverage: 100%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand GOL 14 x GOL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 64.14Å b: 86.11Å c: 118.1Å
α: 90° β: 97.89° γ: 90°
R-values:
R R work R free
0.158 0.157 0.193
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase.
Mu et al. (2014)
1 more