› 4gky

X-ray diffraction
2.42Å resolution

Crystal structure of a carbohydrate-binding domain

Released:
DOI: 10.2210/pdb4gky/pdb
PDB entry 4gky coloured by chain, front view.
PDB entry 4gky coloured by chain, side view.
PDB entry 4gky coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of carbohydrate binding by LMAN1 protein provides new insight into the endoplasmic reticulum export of factors V (FV) and VIII (FVIII).
Zheng C, Page RC, Das V, Nix JC, Wigren E, Misra S, Zhang B
J Biol Chem 288 20499-509 (2013)
PMID: 23709226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein ERGIC-53 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49257 (Residues: 31-270; Coverage: 50%)
Gene names: ERGIC53, F5F8D, LMAN1
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand MAN 1 x MAN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6
Unit cell:
a: 110.263Å b: 110.263Å c: 38.332Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Convergent and divergent mechanisms of sugar recognition across kingdoms.
Taylor et al. (2014)
10 more

2 mentions without citation

Structural characterization of carbohydrate binding by LMAN1 protein provides new insight into the endoplasmic reticulum export of factors V (FV) and VIII (FVIII).
Zheng et al. (2013)
1 more