› 4gni

X-ray diffraction
1.8Å resolution

Structure of the Ssz1 ATPase bound to ATP and Magnesium

Released:
DOI: 10.2210/pdb4gni/pdb
PDB entry 4gni coloured by chain, front view.
PDB entry 4gni coloured by chain, side view.
PDB entry 4gni coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Structural characterization of a eukaryotic chaperone--the ribosome-associated complex.
Leidig C, Bange G, Kopp J, Amlacher S, Aravind A, Wickles S, Witte G, Hurt E, Beckmann R, Sinning I
Nat Struct Mol Biol 20 23-8 (2013)
PMID: 23202586

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-123926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative heat shock protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 44.45 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0RZX9 (Residues: 1-403; Coverage: 70%)
Gene name: CTHT_0008010
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 80.63Å b: 55.38Å c: 98.79Å
α: 90° β: 106.53° γ: 90°
R-values:
R R work R free
0.202 0.201 0.244
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

The endoplasmic reticulum (ER) chaperone BiP is a master regulator of ER functions: Getting by with a little help from ERdj friends.
Pobre et al. (2019)
10 more