› 4go8

X-ray diffraction
2.15Å resolution

Crystal Structure of the TREHALULOSE SYNTHASE MUTB, MUTANT A258V, in complex with TRIS

Released:
DOI: 10.2210/pdb4go8/pdb
PDB entry 4go8 coloured by chain, front view.
PDB entry 4go8 coloured by chain, side view.
PDB entry 4go8 coloured by chain, top view.
Source organism: Burkholderia ubonensis subsp. mesacidophila
Entry authors: Lipski A, Haser R, Aghajari N

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.97 KDa
Source organism: Burkholderia ubonensis subsp. mesacidophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PS28 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 63.51Å b: 71.31Å c: 81.53Å
α: 68.04° β: 72.72° γ: 70.1°
R-values:
R R work R free
0.194 0.19 0.25
Expression system: Escherichia coli

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