› 4gzo

X-ray diffraction
2.6Å resolution

N2 neuraminidase of A/Tanzania/205/2010 H3N2 in complex with hepes

Released:
DOI: 10.2210/pdb4gzo/pdb
PDB entry 4gzo coloured by chain, front view.
PDB entry 4gzo coloured by chain, side view.
PDB entry 4gzo coloured by chain, top view.
Source organism: H3N2 subtype
Primary publication:
Influenza virus neuraminidases with reduced enzymatic activity that avidly bind sialic Acid receptors.
Zhu X, McBride R, Nycholat CM, Yu W, Paulson JC, Wilson IA
J Virol 86 13371-83 (2012)
PMID: 23015718

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 43.35 KDa
Source organism: H3N2 subtype
Expression system: Trichoplusia ni
UniProt:
  • Canonical: K7N5N3 (Residues: 1-393; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand EPE 1 x EPE
Ligand NAG 2 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P4212
Unit cell:
a: 110.091Å b: 110.091Å c: 77.748Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.27
Expression system: Trichoplusia ni

Quick links

Citations

19 review citations

Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement.
Dou et al. (2018)
18 more

8 mentions without citation

NAction! How Can Neuraminidase-Based Immunity Contribute to Better Influenza Virus Vaccines?
Krammer et al. (2018)
7 more