› 4gzw

X-ray diffraction
2.45Å resolution

N2 neuraminidase D151G mutant of A/Tanzania/205/2010 H3N2 in complex with avian sialic acid receptor

Released:
DOI: 10.2210/pdb4gzw/pdb
PDB entry 4gzw coloured by chain, front view.
PDB entry 4gzw coloured by chain, side view.
PDB entry 4gzw coloured by chain, top view.
Source organism: H3N2 subtype
Primary publication:
Influenza virus neuraminidases with reduced enzymatic activity that avidly bind sialic Acid receptors.
Zhu X, McBride R, Nycholat CM, Yu W, Paulson JC, Wilson IA
J Virol 86 13371-83 (2012)
PMID: 23015718

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (8 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 43.29 KDa
Source organism: H3N2 subtype
Expression system: Trichoplusia ni
UniProt:
  • Canonical: K7N5N3 (Residues: 1-393; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, SIA, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, SIA, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: SIA, GAL
Carbohydrate polymer
3 bound ligands:
Ligand NAG 12 x NAG
Ligand CA 5 x CA
Ligand SIA 1 x SIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 82.439Å b: 110.555Å c: 109.928Å
α: 90° β: 97.79° γ: 90°
R-values:
R R work R free
0.199 0.197 0.246
Expression system: Trichoplusia ni

Quick links

Citations

18 review citations

Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement.
Dou et al. (2018)
17 more

4 mentions without citation

Conformations of the type-1 lacto-N-biose I unit in protein complex structures.
Fushinobu S. (2018)
3 more