4h0f

X-ray diffraction
2.4Å resolution

Mutant Structure of laminin-binding adhesin (Lmb) from Streptococcus agalactiae

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-184275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laminin-binding surface protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.59 KDa
Source organism: Streptococcus agalactiae 2603V/R
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DZ80 (Residues: 32-123, 136-306; Coverage: 91%)
Gene names: SAG1234, lmb
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 42.4Å b: 93.99Å c: 66.88Å
α: 90° β: 105.42° γ: 90°
R-values:
R R work R free
0.24 0.24 0.281
Expression system: Escherichia coli BL21(DE3)