› 4h7v

X-ray diffraction
1.8Å resolution

MUTB inactive double mutant D200A-D415N in complex with GLUCOSE

Released:
DOI: 10.2210/pdb4h7v/pdb
PDB entry 4h7v coloured by chain, front view.
PDB entry 4h7v coloured by chain, side view.
PDB entry 4h7v coloured by chain, top view.
Source organism: Rhizobium sp. MX-45
Entry authors: Lipski A, Haser R, Aghajari N

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.89 KDa
Source organism: Rhizobium sp. MX-45
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1F6 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand GLC 2 x GLC
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 63.7Å b: 71.2Å c: 85.4Å
α: 118.1° β: 90.2° γ: 109.7°
R-values:
R R work R free
0.174 0.171 0.221
Expression system: Escherichia coli

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