› 4h8h

X-ray diffraction
2Å resolution

MUTB inactive double mutant E254Q-D415N

Released:
DOI: 10.2210/pdb4h8h/pdb
PDB entry 4h8h coloured by chain, front view.
PDB entry 4h8h coloured by chain, side view.
PDB entry 4h8h coloured by chain, top view.
Source organism: Rhizobium sp. MX-45
Entry authors: Lipski A, Haser R, Aghajari N

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.94 KDa
Source organism: Rhizobium sp. MX-45
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1F6 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 62.93Å b: 84.41Å c: 118.97Å
α: 90° β: 95.94° γ: 90°
R-values:
R R work R free
0.183 0.181 0.228
Expression system: Escherichia coli

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