› 4hap

X-ray diffraction
1.6Å resolution

Crystal Structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with cellobiose

Released:
DOI: 10.2210/pdb4hap/pdb
PDB entry 4hap coloured by chain, front view.
PDB entry 4hap coloured by chain, side view.
PDB entry 4hap coloured by chain, top view.
Source organism: Limnoria quadripunctata
Primary publication:
Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance.
Kern M, McGeehan JE, Streeter SD, Martin RN, Besser K, Elias L, Eborall W, Malyon GP, Payne CM, Himmel ME, Schnorr K, Beckham GT, Cragg SM, Bruce NC, McQueen-Mason SJ
Proc Natl Acad Sci U S A 110 10189-94 (2013)
PMID: 23733951

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Exoglucanase GH7B Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 46.54 KDa
Source organism: Limnoria quadripunctata
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: D4HRL0 (Residues: 19-448; Coverage: 100%)
Gene name: GH7B
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand CA 5 x CA
Ligand TRS 1 x TRS
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 52.26Å b: 54.19Å c: 72.45Å
α: 94.26° β: 96.02° γ: 94.99°
R-values:
R R work R free
0.147 0.144 0.191
Expression system: Aspergillus oryzae

Quick links

Citations

4 review citations

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)
3 more

3 mentions without citation

Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance.
Kern et al. (2013)
2 more