› 4hin

X-ray diffraction
2.4Å resolution

2.4A Resolution Structure of Bovine Cytochrome b5 (S71L)

Released:
DOI: 10.2210/pdb4hin/pdb
PDB entry 4hin coloured by chain, front view.
PDB entry 4hin coloured by chain, side view.
PDB entry 4hin coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Lovell S, Battaile KP, Parthasarathy S, Sun N, Terzyan S, Zhang X, Rivera M, Kuczera K, Benson DR

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 82 amino acids
Theoretical weight: 9.5 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00171 (Residues: 8-89; Coverage: 61%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 47.53Å b: 92.709Å c: 48.99Å
α: 90° β: 113.11° γ: 90°
R-values:
R R work R free
0.21 0.209 0.245
Expression system: Escherichia coli BL21

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