› 4hkz

X-ray diffraction
2.08Å resolution

Trastuzumab Fab complexed with Protein L and Protein A fragments

Released:
DOI: 10.2210/pdb4hkz/pdb
PDB entry 4hkz coloured by chain, front view.
PDB entry 4hkz coloured by chain, side view.
PDB entry 4hkz coloured by chain, top view.
Source organisms:
Primary publication:
Identification and grafting of a unique peptide-binding site in the Fab framework of monoclonal antibodies.
Donaldson JM, Zer C, Avery KN, Bzymek KP, Horne DA, Williams JC
Proc Natl Acad Sci U S A 110 17456-61 (2013)
PMID: 24101516

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-209431 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Trastuzumab light chain Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Structure domains: Immunoglobulins
Trastuzumab heavy chain Chain: B
Molecule details ›
Chain: B
Length: 221 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin G-binding protein A Chain: H
Molecule details ›
Chain: H
Length: 54 amino acids
Theoretical weight: 6.04 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A015 (Residues: 101-151; Coverage: 12%)
Gene names: SAV0111, spa
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C
Gram-positive cocci surface proteins LPxTG domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 63 amino acids
Theoretical weight: 6.85 KDa
Source organism: Finegoldia magna
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51918 (Residues: 476-537; Coverage: 6%)
Sequence domains: Protein L b1 domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 53.52Å b: 104.67Å c: 117.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.206
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

3 review citations

Site-specific antibody-drug conjugates: the nexus of bioorthogonal chemistry, protein engineering, and drug development.
Agarwal et al. (2015)
2 more

7 mentions without citation

Identification and grafting of a unique peptide-binding site in the Fab framework of monoclonal antibodies.
Donaldson et al. (2013)
6 more