› 4ho1

X-ray diffraction
1.86Å resolution

The Crystal structure of Haemophilus influenzae O-acetylserine sulfhydrylase at 1.85A resolution

Released:
DOI: 10.2210/pdb4ho1/pdb
PDB entry 4ho1 coloured by chain, front view.
PDB entry 4ho1 coloured by chain, side view.
PDB entry 4ho1 coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Entry authors: Banerjee S, Singh AK, Kumaran S

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chain: X
Molecule details ›
Chain: X
Length: 316 amino acids
Theoretical weight: 33.65 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P45040 (Residues: 1-316; Coverage: 100%)
Gene names: HI_1103, cysK
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand EDO 2 x EDO
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I41
Unit cell:
a: 112.786Å b: 112.786Å c: 46.297Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.163
Expression system: Escherichia coli

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