› 4hoa

X-ray diffraction
2Å resolution

Crystal structure of the complex of type 1 ribosome inactivating protein from Momordica Balsamina with B-D-galactopyranosyl-(1-4)-D-glucose at 2.0 A resolution

Released:
DOI: 10.2210/pdb4hoa/pdb
PDB entry 4hoa coloured by chain, front view.
PDB entry 4hoa coloured by chain, side view.
PDB entry 4hoa coloured by chain, top view.
Source organism: Momordica balsamina
Entry authors: Yamini S, Pandey N, Kushwaha GS, Sinha M, Kaur P, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
rRNA N-glycosylase Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.09 KDa
Source organism: Momordica balsamina
UniProt:
  • Canonical: D9J2T9 (Residues: 1-246; Coverage: 100%)
Gene name: RIP
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: R3
Unit cell:
a: 130.2Å b: 130.2Å c: 38.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.227

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