› 4hoc

X-ray diffraction
2.2Å resolution

Crystal structure of glucose 1-phosphate thymidylyltransferase from Aneurinibacillus thermoaerophilus complexed with UDP-N-acetylglucosamine

Released:
DOI: 10.2210/pdb4hoc/pdb
PDB entry 4hoc coloured by chain, front view.
PDB entry 4hoc coloured by chain, side view.
PDB entry 4hoc coloured by chain, top view.
Source organism: Aneurinibacillus thermoaerophilus
Entry authors: Chen TJ, Chien WT, Lin CC, Wang WC

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate thymidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 32.97 KDa
Source organism: Aneurinibacillus thermoaerophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AGY4 (Residues: 1-296; Coverage: 100%)
Gene name: rmlA
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand UD1 2 x UD1
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: C2
Unit cell:
a: 156.308Å b: 70.309Å c: 89.374Å
α: 90° β: 124.85° γ: 90°
R-values:
R R work R free
0.207 0.205 0.248
Expression system: Escherichia coli

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