› 4hs6

X-ray diffraction
1.53Å resolution

Hepatitus C envelope glycoprotein E2 fragment 412-423 with humanized and affinity-matured antibody MRCT10.v362

Released:
DOI: 10.2210/pdb4hs6/pdb
PDB entry 4hs6 coloured by chain, front view.
PDB entry 4hs6 coloured by chain, side view.
PDB entry 4hs6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies.
Pantua H, Diao J, Ultsch M, Hazen M, Mathieu M, McCutcheon K, Takeda K, Date S, Cheung TK, Phung Q, Hass P, Arnott D, Hongo JA, Matthews DJ, Brown A, Patel AH, Kelley RF, Eigenbrot C, Kapadia SB
J Mol Biol 425 1899-1914 (2013)
PMID: 23458406

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212899 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
E2-peptide Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 16 amino acids
Theoretical weight: 1.73 KDa
UniProt:
  • Canonical: Q9YK84 (Residues: 51-62; Coverage: 9%)
MRCT10.v362 Fab light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 218 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
MRCT10.v362 Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 226 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 71.091Å b: 90.821Å c: 72.681Å
α: 90° β: 109.35° γ: 90°
R-values:
R R work R free
0.175 0.175 0.2
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The past, present and future of neutralizing antibodies for hepatitis C virus.
Ball et al. (2014)
30 more

7 mentions without citation

Computational Modeling of Hepatitis C Virus Envelope Glycoprotein Structure and Recognition.
Guest et al. (2018)
6 more