› 4hs8

X-ray diffraction
2.6Å resolution

Hepatitus C envelope glycoprotein E2 fragment 412-423 with humanized and affinity-matured antibody hu5B3.v3

Released:
DOI: 10.2210/pdb4hs8/pdb
PDB entry 4hs8 coloured by chain, front view.
PDB entry 4hs8 coloured by chain, side view.
PDB entry 4hs8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies.
Pantua H, Diao J, Ultsch M, Hazen M, Mathieu M, McCutcheon K, Takeda K, Date S, Cheung TK, Phung Q, Hass P, Arnott D, Hongo JA, Matthews DJ, Brown A, Patel AH, Kelley RF, Eigenbrot C, Kapadia SB
J Mol Biol 425 1899-1914 (2013)
PMID: 23458406

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212900 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
E2-peptide Chain: A
Molecule details ›
Chain: A
Length: 16 amino acids
Theoretical weight: 1.73 KDa
UniProt:
  • Canonical: Q9YK84 (Residues: 51-62; Coverage: 9%)
antibody hu5B3.v2 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody hu5B3.v2 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 77.716Å b: 77.716Å c: 181.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.264
Expression system: Escherichia coli

Quick links

Citations

32 review citations

The past, present and future of neutralizing antibodies for hepatitis C virus.
Ball et al. (2014)
31 more

5 mentions without citation

Conformational Flexibility in the CD81-Binding Site of the Hepatitis C Virus Glycoprotein E2.
Ströh et al. (2018)
4 more