› 4hzx

X-ray diffraction
2.2Å resolution

Crystal structure of influenza A neuraminidase N3 complexed with oseltamivir

Released:
DOI: 10.2210/pdb4hzx/pdb
PDB entry 4hzx coloured by chain, front view.
PDB entry 4hzx coloured by chain, side view.
PDB entry 4hzx coloured by chain, top view.
Source organism: Influenza A virus (A/swine/Missouri/2124514/2006(H2N3))
Primary publication:
Functional and structural analysis of influenza virus neuraminidase N3 offers further insight into the mechanisms of oseltamivir resistance.
Li Q, Qi J, Wu Y, Kiyota H, Tanaka K, Suhara Y, Ohrui H, Suzuki Y, Vavricka CJ, Gao GF
J Virol 87 10016-24 (2013)
PMID: 23824808

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.33 KDa
Source organism: Influenza A virus (A/swine/Missouri/2124514/2006(H2N3))
Expression system: Not provided
UniProt:
  • Canonical: A9YN63 (Residues: 83-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand G39 1 x G39
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: I4
Unit cell:
a: 106.224Å b: 106.224Å c: 66.448Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.197
Expression system: Not provided

Quick links

Citations

4 review citations

Bat-derived influenza-like viruses H17N10 and H18N11.
Wu et al. (2014)
3 more

1 mention without citation

Functional and structural analysis of influenza virus neuraminidase N3 offers further insight into the mechanisms of oseltamivir resistance.
Li et al. (2013)