› 4i5d

X-ray diffraction
2.4Å resolution

Crystal structure of Ralstonia sp. alcohol dehydrogenase in its apo form

Released:
DOI: 10.2210/pdb4i5d/pdb
PDB entry 4i5d coloured by chain, front view.
PDB entry 4i5d coloured by chain, side view.
PDB entry 4i5d coloured by chain, top view.
Source organism: Ralstonia sp. DSMZ 6428
Primary publication:
Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity.
Lerchner A, Jarasch A, Meining W, Schiefner A, Skerra A
Biotechnol Bioeng 110 2803-14 (2013)
PMID: 23686719

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alclohol dehydrogenase/short-chain dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 262 amino acids
Theoretical weight: 28.18 KDa
Source organism: Ralstonia sp. DSMZ 6428
Expression system: Escherichia coli
UniProt:
  • Canonical: C0IR58 (Residues: 2-249; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 73.074Å b: 73.518Å c: 132.677Å
α: 80.61° β: 86.8° γ: 63.1°
R-values:
R R work R free
0.197 0.195 0.231
Expression system: Escherichia coli

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Citations

2 review citations

Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals.
Mehtiö et al. (2016)
1 more