4i7b

X-ray diffraction
3Å resolution

Siah1 bound to synthetic peptide (ACE)KLRPV(ABA)MVRPTVR

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-173294 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SIAH1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 196 amino acids
Theoretical weight: 21.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IUQ4 (Residues: 90-282; Coverage: 68%)
Gene names: HUMSIAH, SIAH1
Sequence domains:
Structure domains:
Protein phyllopod Chains: B, D
Molecule details ›
Chains: B, D
Length: 14 amino acids
Theoretical weight: 1.57 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: Q27934 (Residues: 112-125; Coverage: 4%)
Gene names: CG10108, phyl

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 39.745Å b: 87.913Å c: 59.884Å
α: 90° β: 100.59° γ: 90°
R-values:
R R work R free
0.19 0.186 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided