› 4iag

X-ray diffraction
1.9Å resolution

Crystal structure of ZbmA, the zorbamycin binding protein from Streptomyces flavoviridis

Released:
DOI: 10.2210/pdb4iag/pdb
PDB entry 4iag coloured by chain, front view.
PDB entry 4iag coloured by chain, side view.
PDB entry 4iag coloured by chain, top view.
Source organism: Streptomyces pilosus
Primary publication:
Crystal Structure of the Zorbamycin-Binding Protein ZbmA, the Primary Self-Resistance Element in Streptomyces flavoviridis ATCC21892.
Rudolf JD, Bigelow L, Chang C, Cuff ME, Lohman JR, Chang CY, Ma M, Yang D, Clancy S, Babnigg G, Joachimiak A, Phillips GN, Shen B
Biochemistry 54 6842-51 (2015)
PMID: 26512730

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110893 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zbm binding protein Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.02 KDa
Source organism: Streptomyces pilosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9UIZ4 (Residues: 1-132; Coverage: 100%)
Gene name: zbmA
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:
Ligand EDO 8 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6522
Unit cell:
a: 38.787Å b: 38.787Å c: 268.524Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Antibiotic Resistance Mechanisms in Bacteria: Relationships Between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens.
Peterson et al. (2018)
4 more

3 mentions without citation

Crystal Structure of the Zorbamycin-Binding Protein ZbmA, the Primary Self-Resistance Element in Streptomyces flavoviridis ATCC21892.
Rudolf et al. (2015)
2 more