› 4iay

X-ray diffraction
2Å resolution

Room temperature X-ray Structure of cAMP dependent protein kinase A in complex with high Sr2+ concentration, ADP and phosphorylated peptide pSP20

Released:
DOI: 10.2210/pdb4iay/pdb
PDB entry 4iay coloured by chain, front view.
PDB entry 4iay coloured by chain, side view.
PDB entry 4iay coloured by chain, top view.
Source organisms:
Primary publication:
Insights into the phosphoryl transfer catalyzed by cAMP-dependent protein kinase: an X-ray crystallographic study of complexes with various metals and peptide substrate SP20.
Gerlits O, Waltman MJ, Taylor S, Langan P, Kovalevsky A
OpenAccess logo Biochemistry 52 3721-7 (2013)
PMID: 23672593

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138544 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 41.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: S
Molecule details ›
Chain: S
Length: 20 amino acids
Theoretical weight: 2.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61925 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

2 bound ligands:
Ligand SR 2 x SR
Ligand ADP 1 x ADP
2 modified residues:
Ligand SEP 3 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 59.484Å b: 80.014Å c: 100.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Molecular mechanisms of asymmetric RAF dimer activation.
Jambrina et al. (2014)
15 other articles

2 mentions without citation

Insights into the phosphoryl transfer catalyzed by cAMP-dependent protein kinase: an X-ray crystallographic study of complexes with various metals and peptide substrate SP20.
Gerlits et al. (2013)
1 more