› 4if7

X-ray diffraction
2Å resolution

Mycobacterium Tuberculosis Methionine aminopeptidase Type 1c in complex with homocysteine-methyl disulfide

Released:
DOI: 10.2210/pdb4if7/pdb
Model geometry
Fit model/data
PDB entry 4if7 coloured by chain, front view.
PDB entry 4if7 coloured by chain, side view.
PDB entry 4if7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Selective targeting of the conserved active site cysteine of Mycobacterium tuberculosis methionine aminopeptidase with electrophilic reagents.
Reddi R, Arya T, Kishor C, Gumpena R, Ganji RJ, Bhukya S, Addlagatta A
FEBS J 281 4240-8 (2014)
PMID: 24841365

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase 2 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.8 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WK19 (Residues: 1-285; Coverage: 100%)
Gene names: MTV003.07c, Rv2861c, map, mapB
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand HCM 1 x HCM
1 modified residue:
Ligand SCH 1 x SCH

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 49.38Å b: 48.216Å c: 56.4Å
α: 90° β: 95.25° γ: 90°
R-values:
R R work R free
0.205 0.201 0.266
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent advancements in the development of anti-tuberculosis drugs.
Chetty et al. (2017)
4 other articles

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO