› 4ilt

X-ray diffraction
2.55Å resolution

Structure of the dioxygenase domain of SACTE_2871, a novel dioxygenase carbohydrate-binding protein fusion from the cellulolytic bacterium Streptomyces sp. SirexAA-E

Released:
DOI: 10.2210/pdb4ilt/pdb
PDB entry 4ilt coloured by chain, front view.
PDB entry 4ilt coloured by chain, side view.
PDB entry 4ilt coloured by chain, top view.
Source organism: Streptomyces sp. SirexAA-E
Primary publication:
Fusion of dioxygenase and lignin-binding domains in a novel secreted enzyme from cellulolytic Streptomyces sp. SirexAA-E.
Bianchetti CM, Harmann CH, Takasuka TE, Hura GL, Dyer K, Fox BG
J Biol Chem 288 18574-87 (2013)
PMID: 23653358

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitin-binding type-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 16.69 KDa
Source organism: Streptomyces sp. SirexAA-E
Expression system: Escherichia coli
UniProt:
  • Canonical: G2NEL6 (Residues: 77-230; Coverage: 53%)
Gene name: SACTE_2871
Sequence domains: Dioxygenase
Structure domains: Aromatic compound dioxygenase

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 49.192Å b: 85.125Å c: 70.264Å
α: 90° β: 95.53° γ: 90°
R-values:
R R work R free
0.207 0.203 0.268
Expression system: Escherichia coli

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Citations

4 review citations

Exploring bacterial lignin degradation.
Brown et al. (2014)
3 more

2 mentions without citation

Fusion of dioxygenase and lignin-binding domains in a novel secreted enzyme from cellulolytic Streptomyces sp. SirexAA-E.
Bianchetti et al. (2013)
1 more