› 4im7

X-ray diffraction
1.9Å resolution

Crystal structure of fructuronate reductase (ydfI) from E. coli CFT073 (EFI TARGET EFI-506389) complexed with NADH and D-mannonate

Released:
DOI: 10.2210/pdb4im7/pdb
PDB entry 4im7 coloured by chain, front view.
PDB entry 4im7 coloured by chain, side view.
PDB entry 4im7 coloured by chain, top view.
Source organism: Escherichia coli CFT073
Entry authors: Lukk T, Wichelecki D, Imker HJ, Gerlt JA, Nair SK

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypothetical oxidoreductase ydfI Chain: A
Molecule details ›
Chain: A
Length: 506 amino acids
Theoretical weight: 55.93 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2V7F2 (Residues: 1-486; Coverage: 100%)
Gene name: c1968
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CS2 1 x CS2
Ligand NAI 1 x NAI
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 92.49Å b: 92.49Å c: 136Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.148 0.173
Expression system: Escherichia coli BL21(DE3)

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