4iol

X-ray diffraction
2.56Å resolution

N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with ADP/ZD9 and XPO

Released:

Function and Biology Details

Reaction catalysed:
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-173900 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate--tetrahydrofolate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 60.01 KDa
Source organism: Moorella thermoacetica ATCC 39073
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2RM91 (Residues: 1-559; Coverage: 100%)
Gene names: Moth_0109, fhs
Sequence domains: Formate--tetrahydrofolate ligase
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R32
Unit cell:
a: 160.37Å b: 160.37Å c: 258.074Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.19 0.279
Expression system: Escherichia coli