› 4ira

X-ray diffraction
2.2Å resolution

CobR in complex with FAD

Released:
DOI: 10.2210/pdb4ira/pdb
PDB entry 4ira coloured by chain, front view.
PDB entry 4ira coloured by chain, side view.
PDB entry 4ira coloured by chain, top view.
Source organism: Brucella melitensis bv. 1 str. 16M
Entry authors: Lawrence AD, Scott AF, Warren MJ, Pickersgill RW

Function and Biology Details

Reaction catalysed: 
4-hydroxyphenylacetate + FADH(2) + O(2) = 3,4-dihydroxyphenylacetate + FAD + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin reductase like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 18.75 KDa
Source organism: Brucella melitensis bv. 1 str. 16M
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8YHT7 (Residues: 1-173; Coverage: 100%)
Gene name: BMEI0709
Sequence domains: Flavin reductase like domain
Structure domains: Electron Transport, Fmn-binding Protein; Chain A

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P6222
Unit cell:
a: 103.193Å b: 103.193Å c: 133.103Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.25
Expression system: Escherichia coli BL21

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