› 4j1j

X-ray diffraction
2.65Å resolution

Leanyer orthobunyavirus nucleoprotein-ssDNA complex

Released:
DOI: 10.2210/pdb4j1j/pdb
PDB entry 4j1j coloured by chain, front view.
PDB entry 4j1j coloured by chain, side view.
PDB entry 4j1j coloured by chain, top view.
Source organism: Leanyer virus
Primary publication:
Structure of the Leanyer orthobunyavirus nucleoprotein-RNA complex reveals unique architecture for RNA encapsidation.
Niu F, Shaw N, Wang YE, Jiao L, Ding W, Li X, Zhu P, Upur H, Ouyang S, Cheng G, Liu ZJ
Proc Natl Acad Sci U S A 110 9054-9 (2013)
PMID: 23569220

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117672 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.3 KDa
Source organism: Leanyer virus
Expression system: Escherichia coli
UniProt:
  • Canonical: F2WAD7 (Residues: 1-235; Coverage: 100%)
Sequence domains: Bunyavirus nucleocapsid (N) protein
Structure domains:
DNA (5'-D(P*AP*CP*CP*AP*AP*AP*CP*AP*AP*CP*CP*CP*AP*CP*CP*CP*A)-3') Chains: H, I
Molecule details ›
Chains: H, I
Length: 17 nucleotides
Theoretical weight: 5.06 KDa
Source organism: Leanyer virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 50.403Å b: 73.199Å c: 77.905Å
α: 70.89° β: 82.54° γ: 73.73°
R-values:
R R work R free
0.224 0.223 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Orthobunyaviruses: recent genetic and structural insights.
Elliott RM. (2014)
11 more

7 mentions without citation

Multiple origins of viral capsid proteins from cellular ancestors.
Krupovic et al. (2017)
6 more