› 4j27

X-ray diffraction
1.59Å resolution

Crystal structure of a gh29 alpha-l-fucosidase gh29 from bacteroides thetaiotaomicron in a novel crystal form

Released:
DOI: 10.2210/pdb4j27/pdb
PDB entry 4j27 coloured by chain, front view.
PDB entry 4j27 coloured by chain, side view.
PDB entry 4j27 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Three dimensional structure of a bacterial α-l-fucosidase with a 5-membered iminocyclitol inhibitor.
Wright DW, Moreno-Vargas AJ, Carmona AT, Robina I, Davies GJ
Bioorg Med Chem 21 4751-4 (2013)
PMID: 23830696

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 450 amino acids
Theoretical weight: 52.08 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A3I4 (Residues: 35-484; Coverage: 99%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand IMD 5 x IMD
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 68.15Å b: 96.55Å c: 97.27Å
α: 90° β: 91.3° γ: 90°
R-values:
R R work R free
0.159 0.158 0.178
Expression system: Escherichia coli BL21

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Citations

6 citation in other articles

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia.
Jiménez et al. (2014)
5 more

5 mentions without citation

Rapid method for isolation of desiccation-tolerant strains and xeroprotectants.
Narváez-Reinaldo et al. (2010)
4 more