› 4jim

X-ray diffraction
2.1Å resolution

Native Crystal Structure of N10-Formyltetrahydrofolate Synthetase

Released:
DOI: 10.2210/pdb4jim/pdb
PDB entry 4jim coloured by chain, front view.
PDB entry 4jim coloured by chain, side view.
PDB entry 4jim coloured by chain, top view.
Source organism: Moorella thermoacetica ATCC 39073
Primary publication:
Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.
Celeste LR, Chai G, Bielak M, Minor W, Lovelace LL, Lebioda L
Protein Sci 21 219-28 (2012)
PMID: 22109967

Function and Biology Details

Reaction catalysed: 
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-173899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate--tetrahydrofolate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 60.01 KDa
Source organism: Moorella thermoacetica ATCC 39073
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2RM91 (Residues: 1-559; Coverage: 100%)
Gene names: Moth_0109, fhs
Sequence domains: Formate--tetrahydrofolate ligase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TOE 3 x TOE
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R32
Unit cell:
a: 161.192Å b: 161.192Å c: 256.903Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.274
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Early bioenergetic evolution.
Sousa et al. (2013)
2 more

1 mention without citation

Response to Boguslaw Stec's letter to the editor of Protein Science.
Celeste et al. (2013)