› 4jjk

X-ray diffraction
3Å resolution

Crystal Structure of N10-Formyltetrahydrofolate Synthetase with Folate

Released:
DOI: 10.2210/pdb4jjk/pdb
PDB entry 4jjk coloured by chain, front view.
PDB entry 4jjk coloured by chain, side view.
PDB entry 4jjk coloured by chain, top view.
Source organism: Moorella thermoacetica ATCC 39073
Primary publication:
Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.
Celeste LR, Chai G, Bielak M, Minor W, Lovelace LL, Lebioda L
Protein Sci 21 219-28 (2012)
PMID: 22109967

Function and Biology Details

Reaction catalysed: 
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate--tetrahydrofolate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 60.01 KDa
Source organism: Moorella thermoacetica ATCC 39073
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2RM91 (Residues: 1-559; Coverage: 100%)
Gene names: Moth_0109, fhs
Sequence domains: Formate--tetrahydrofolate ligase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FOL 1 x FOL
Ligand EDO 3 x EDO
Ligand TOE 3 x TOE
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R32
Unit cell:
a: 160.948Å b: 160.948Å c: 256.532Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.21 0.286
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Early bioenergetic evolution.
Sousa et al. (2013)
2 more

2 mentions without citation

Response to Boguslaw Stec's letter to the editor of Protein Science.
Celeste et al. (2013)
1 more