› 4k1k

X-ray diffraction
1.6Å resolution

Induced opening of influenza virus neuraminidase N2 150-loop suggests an important role in inhibitor binding

Released:
DOI: 10.2210/pdb4k1k/pdb
PDB entry 4k1k coloured by chain, front view.
PDB entry 4k1k coloured by chain, side view.
PDB entry 4k1k coloured by chain, top view.
Source organism: Influenza A virus (A/RI/5+/1957(H2N2))
Primary publication:
Induced opening of influenza virus neuraminidase N2 150-loop suggests an important role in inhibitor binding.
Wu Y, Qin G, Gao F, Liu Y, Vavricka CJ, Qi J, Jiang H, Yu K, Gao GF
OpenAccess logo Sci Rep 3 1551 (2013)
PMID: 23531861

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.05 KDa
Source organism: Influenza A virus (A/RI/5+/1957(H2N2))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q194T1 (Residues: 82-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand G39 2 x G39
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 114.912Å b: 139.358Å c: 140.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.19
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Novel antiviral drug discovery strategies to tackle drug-resistant mutants of influenza virus strains.
Shin et al. (2019)
1 more

4 mentions without citation

Structure of influenza virus N7: the last piece of the neuraminidase "jigsaw" puzzle.
Sun et al. (2014)
3 more