› 4k3s

X-ray diffraction
1.75Å resolution

E. coli sliding clamp in P1 crystal space group

Released:
DOI: 10.2210/pdb4k3s/pdb
PDB entry 4k3s coloured by chain, front view.
PDB entry 4k3s coloured by chain, side view.
PDB entry 4k3s coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and thermodynamic dissection of linear motif recognition by the E. coli sliding clamp.
Yin Z, Kelso MJ, Beck JL, Oakley AJ
J Med Chem 56 8665-73 (2013)
PMID: 24090409

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 40.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand PG4 1 x PG4
Ligand P4C 1 x P4C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 40.98Å b: 64.97Å c: 71.93Å
α: 73.81° β: 82.46° γ: 84.04°
R-values:
R R work R free
0.197 0.195 0.229
Expression system: Escherichia coli

Quick links

Citations

1 review citation

DNA Sliding Clamps as Therapeutic Targets.
Altieri et al. (2018)
10 other articles

1 mention without citation

Predicting binding sites from unbound versus bound protein structures.
Clark et al. (2020)