› 4k6c

X-ray diffraction
1.85Å resolution

X-ray crystal structure of a putative Acetoacyl-CoA reductase from Burkholderia cenocepacia

Released:
DOI: 10.2210/pdb4k6c/pdb
PDB entry 4k6c coloured by chain, front view.
PDB entry 4k6c coloured by chain, side view.
PDB entry 4k6c coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetoacetyl-CoA reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.45 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EAU4 (Residues: 1-247; Coverage: 100%)
Gene names: BCAL0833, phbB
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 84.48Å b: 84.48Å c: 143.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.196
Expression system: Escherichia coli

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