› 4k7m

X-ray diffraction
1.8Å resolution

Crystal structure of RNase S variant (K7C/Q11C) with bound mercury ions

Released:
DOI: 10.2210/pdb4k7m/pdb
PDB entry 4k7m coloured by chain, front view.
PDB entry 4k7m coloured by chain, side view.
PDB entry 4k7m coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal Structure of Apo- and Metalated Thiolate containing RNase S as Structural Basis for the Design of Artificial Metalloenzymes by Peptide- Protein Complementation
Genz M, Singer D, Hey-Hawkins E, Hoffmann R, Straeter N
Z.Anorg.Allg.Chem. 639 2395-2400 (2013)

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.24 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 96%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand HG 2 x HG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P3121
Unit cell:
a: 43.87Å b: 43.87Å c: 96.361Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.205
Expression system: Not provided

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