› 4kbj

X-ray diffraction
2.45Å resolution

Structure of Mtb RNAP Beta subunit B1 and B2 domains

Released:
DOI: 10.2210/pdb4kbj/pdb
PDB entry 4kbj coloured by chain, front view.
PDB entry 4kbj coloured by chain, side view.
PDB entry 4kbj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of the Mtb CarD/RNAP β-lobes complex reveals the molecular basis of interaction and presents a distinct DNA-binding domain for Mtb CarD.
Gulten G, Sacchettini JC
Structure 21 1859-69 (2013)
PMID: 24055315

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 46.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY9 (Residues: 53-439; Coverage: 33%)
Gene names: MTCI376.08c, Rv0667, rpoB
Sequence domains:
Structure domains: RNA polymerase Rpb2, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 53.183Å b: 123.911Å c: 135.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.263
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mycobacterium tuberculosis Transcription Machinery: Ready To Respond to Host Attacks.
Flentie et al. (2016)
4 more

3 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
2 more