› 4kgi

X-ray diffraction
1.6Å resolution

Crystal structure of a glutathione transferase family member from Shigella flexneri, target EFI-507258, bound GSH, TEV-his-tag linker in active site

Released:
DOI: 10.2210/pdb4kgi/pdb
PDB entry 4kgi coloured by chain, front view.
PDB entry 4kgi coloured by chain, side view.
PDB entry 4kgi coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 2457T
Entry authors: Vetting MW, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Stead M, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Armstrong RN, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 25.46 KDa
Source organism: Shigella flexneri 2a str. 2457T
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GSH 4 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 57.849Å b: 63.964Å c: 64.238Å
α: 94.41° β: 103.23° γ: 92.11°
R-values:
R R work R free
0.178 0.176 0.21
Expression system: Escherichia coli

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