4kzv

X-ray diffraction
1.4Å resolution

Structure of the carbohydrate-recognition domain of the C-type lectin mincle bound to trehalose

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-122748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
C-type lectin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.74 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E1BHM0 (Residues: 79-211; Coverage: 63%)
Gene name: CLEC4E
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 73.48Å b: 73.48Å c: 99.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.207
Expression system: Escherichia coli BL21(DE3)