› 4l5e

X-ray diffraction
1.34Å resolution

Crystal structure of A. aeolicus NtrC1 DNA binding domain

Released:
DOI: 10.2210/pdb4l5e/pdb
PDB entry 4l5e coloured by chain, front view.
PDB entry 4l5e coloured by chain, side view.
PDB entry 4l5e coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structure, function, and tethering of DNA-binding domains in σ⁵⁴ transcriptional activators.
Vidangos N, Maris AE, Young A, Hong E, Pelton JG, Batchelor JD, Wemmer DE
Biopolymers 99 1082-96 (2013)
PMID: 23818155

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator (NtrC family) Chain: A
Molecule details ›
Chain: A
Length: 46 amino acids
Theoretical weight: 5.47 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 393-438; Coverage: 11%)
Gene names: aq_1117, ntrC1
Sequence domains: Bacterial regulatory protein, Fis family
Structure domains: Homeodomain-like

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 33.378Å b: 62.268Å c: 54.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.206
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial Enhancer Binding Proteins-AAA+ Proteins in Transcription Activation.
Gao et al. (2020)
10 other articles

3 mentions without citation

Mechanistic insights into c-di-GMP-dependent control of the biofilm regulator FleQ from Pseudomonas aeruginosa.
Matsuyama et al. (2016)
2 more