› 4l5i

X-ray diffraction
3.21Å resolution

Crystal structures of the LsrR proteins complexed with phospho-AI-2 and its two different analogs reveal distinct mechanisms for ligand recognition

Released:
DOI: 10.2210/pdb4l5i/pdb
PDB entry 4l5i coloured by chain, front view.
PDB entry 4l5i coloured by chain, side view.
PDB entry 4l5i coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and two signal-interrupting analogues reveal distinct mechanisms for ligand recognition.
Ha JH, Eo Y, Grishaev A, Guo M, Smith JA, Sintim HO, Kim EH, Cheong HK, Bentley WE, Ryu KS
J Am Chem Soc 135 15526-35 (2013)
PMID: 24047255

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-160057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator LsrR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 318 amino acids
Theoretical weight: 33.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76141 (Residues: 1-317; Coverage: 100%)
Gene names: JW1505, b1512, lsrR, ydeW
Sequence domains: Putative sugar-binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 176.744Å b: 80.535Å c: 103.635Å
α: 90° β: 112.18° γ: 90°
R-values:
R R work R free
0.226 0.226 0.287
Expression system: Escherichia coli

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Citations

2 review citations

Mechanisms of Inhibition of Quorum Sensing as an Alternative for the Control of E. coli and Salmonella.
Escobar-Muciño et al. (2022)
1 more

1 mention without citation

Crystal structures of the LsrR proteins complexed with phospho-AI-2 and two signal-interrupting analogues reveal distinct mechanisms for ligand recognition.
Ha et al. (2013)