› 4l78

X-ray diffraction
2.18Å resolution

Xenon Trapping and Statistical Coupling Analysis Uncover Regions Important for Structure and Function of Multidomain Protein StPurL

Released:
DOI: 10.2210/pdb4l78/pdb
PDB entry 4l78 coloured by chain, front view.
PDB entry 4l78 coloured by chain, side view.
PDB entry 4l78 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Importance of hydrophobic cavities in allosteric regulation of formylglycinamide synthetase: insight from xenon trapping and statistical coupling analysis.
Tanwar AS, Goyal VD, Choudhary D, Panjikar S, Anand R
OpenAccess logo PLoS One 8 e77781 (2013)
PMID: 24223728

Function and Biology Details

Reaction catalysed: 
ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylformylglycinamidine synthase Chain: A
Molecule details ›
Chain: A
Length: 1303 amino acids
Theoretical weight: 142.48 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P74881 (Residues: 1-1295; Coverage: 100%)
Gene names: STM2565, purL
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 4 x MG
Ligand MN 1 x MN
Ligand SO4 12 x SO4
Ligand XE 5 x XE
Ligand CL 3 x CL
Ligand ACT 2 x ACT
1 modified residue:
Ligand CYG 1 x CYG

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P65
Unit cell:
a: 146.68Å b: 146.68Å c: 141.221Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.139 0.188
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Higher order structures in purine and pyrimidine metabolism.
Chitrakar et al. (2017)
4 other articles

1 mention without citation

Importance of hydrophobic cavities in allosteric regulation of formylglycinamide synthetase: insight from xenon trapping and statistical coupling analysis.
Tanwar et al. (2013)