› 4lbv

X-ray diffraction
2.03Å resolution

Identifying ligand binding hot spots in proteins using brominated fragments

Released:
DOI: 10.2210/pdb4lbv/pdb
PDB entry 4lbv coloured by chain, front view.
PDB entry 4lbv coloured by chain, side view.
PDB entry 4lbv coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Identifying ligand-binding hot spots in proteins using brominated fragments.
Grøftehauge MK, Therkelsen MØ, Taaning R, Skrydstrup T, Morth JP, Nissen P
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 1060-5 (2013)
PMID: 23989163

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-157997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor Tu-A Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 44.61 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60338 (Residues: 3-406; Coverage: 100%)
Gene names: tuf, tufA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand NH4 1 x NH4
Ligand CL 1 x CL
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: C2
Unit cell:
a: 147.38Å b: 98.61Å c: 39.78Å
α: 90° β: 95.97° γ: 90°
R-values:
R R work R free
0.163 0.162 0.193
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit.
Dongre et al. (2016)
2 more

2 mentions without citation

Identifying ligand-binding hot spots in proteins using brominated fragments.
Grøftehauge et al. (2013)
1 more