› 4lcq

X-ray diffraction
1.81Å resolution

The crystal structure of di-Zn dihydropyrimidinase in complex with NCBI

Released:
DOI: 10.2210/pdb4lcq/pdb
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PDB entry 4lcq coloured by chain, front view.
PDB entry 4lcq coloured by chain, side view.
PDB entry 4lcq coloured by chain, top view.
Source organism: Tetraodon nigroviridis
Primary publication:
Crystal structures of vertebrate dihydropyrimidinase and complexes from Tetraodon nigroviridis with lysine carbamylation: metal and structural requirements for post-translational modification and function.
Hsieh YC, Chen MC, Hsu CC, Chan SI, Yang YS, Chen CJ
J Biol Chem 288 30645-30658 (2013)
PMID: 24005677

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 56.93 KDa
Source organism: Tetraodon nigroviridis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4SMR4 (Residues: 1-500; Coverage: 100%)
Gene name: GSTENG00015639001
Sequence domains: Amidohydrolase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand URQ 1 x URQ
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: I4122
Unit cell:
a: 161.204Å b: 161.204Å c: 93.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.2
Expression system: Escherichia coli

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Citations

2 review citations

In Vitro Assessment of Fluoropyrimidine-Metabolizing Enzymes: Dihydropyrimidine Dehydrogenase, Dihydropyrimidinase, and β-Ureidopropionase.
Hishinuma et al. (2020)
1 more

1 mention without citation

Crystal structures of vertebrate dihydropyrimidinase and complexes from Tetraodon nigroviridis with lysine carbamylation: metal and structural requirements for post-translational modification and function.
Hsieh et al. (2013)

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