› 4lf3

X-ray diffraction
2.74Å resolution

Inhibitory Mechanism of an Allosteric Antibody Targeting the Glucagon Receptor

Released:
DOI: 10.2210/pdb4lf3/pdb
PDB entry 4lf3 coloured by chain, front view.
PDB entry 4lf3 coloured by chain, side view.
PDB entry 4lf3 coloured by chain, top view.
Source organisms:
Primary publication:
Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor.
Mukund S, Shang Y, Clarke HJ, Madjidi A, Corn JE, Kates L, Kolumam G, Chiang V, Luis E, Murray J, Zhang Y, Hötzel I, Koth CM, Allan BB
OpenAccess logo J Biol Chem 288 36168-78 (2013)
PMID: 24189067

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211262 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab heavy chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 214 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Fab light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 231 amino acids
Theoretical weight: 25 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Glucagon receptor Chains: C, F
Molecule details ›
Chains: C, F
Length: 95 amino acids
Theoretical weight: 11.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47871 (Residues: 29-123; Coverage: 21%)
Gene name: GCGR
Sequence domains: Hormone receptor domain
Structure domains: GPCR, family 2, extracellular hormone receptor domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 104.928Å b: 62.777Å c: 116.432Å
α: 90° β: 106.04° γ: 90°
R-values:
R R work R free
0.212 0.209 0.264
Expression systems:
  • Escherichia coli BL21
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

Mechanisms of signalling and biased agonism in G protein-coupled receptors.
Wootten et al. (2018)
3 more

6 mentions without citation

Transmembrane signal transduction by peptide hormones via family B G protein-coupled receptors.
Culhane et al. (2015)
5 more