› 4lih

X-ray diffraction
1.85Å resolution

The crystal structure of Gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase from Burkholderia cenocepacia J2315

Released:
DOI: 10.2210/pdb4lih/pdb
PDB entry 4lih coloured by chain, front view.
PDB entry 4lih coloured by chain, side view.
PDB entry 4lih coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 504 amino acids
Theoretical weight: 53.91 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4E926 (Residues: 1-496; Coverage: 100%)
Gene names: BCAL0603, puuC
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MES 8 x MES
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 90.31Å b: 104.19Å c: 108.28Å
α: 91.7° β: 99.04° γ: 90.02°
R-values:
R R work R free
0.191 0.189 0.232
Expression system: Escherichia coli

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